LOCUS       pWJ402                  7710 bp    DNA     circular SYN 18-FEB-2020
DEFINITION  .
ACCESSION   pWJ402
KEYWORDS    .
SOURCE      Unknown.
  ORGANISM  Unknown
            Unclassified.
REFERENCE   1  (bases 1 to 7710)
  AUTHORS   Self
  JOURNAL   Unpublished.
COMMENT     SECID/File created by Clone Manager, Scientific & Educational Software
FEATURES             Location/Qualifiers
     CDS             172..819
                     /codon_start=1
                     /gene="cat"
                     /note="cat"
                     /translation="MNFNKIDLDNWKRKEIFNHYLNQQTTFSITTEIDISVLYRNIKQ
                     EGYKFYPAFIFLVTRVINSNTAFRTGYNSDGELGYWDKLEPLYTIFDGVSKTFSGIWT
                     PVKNDFKEFYDLYLSDVEKYNGSGKLFPKTPIPENAFSLSIIPWTSFTGFNLNINNNS
                     NYLLPIITAGKFINKGNSIYLPLSLQVHHSVCDGYHAGLFMNSIQELSDRPNDWLL"
     CDS             complement(1552..2238)
                     /gene="rep"
                     /note="rep"
     misc_feature    complement(2783..2871)
                     /gene="tracr"
     CDS             3112..7215
                     /codon_start=1
                     /gene="cas9"
                     /note="cas9"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIK
                     KNLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLE
                     ESFLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAH
                     MIKFRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSK
                     SRRLENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLD
                     NLLAQIGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTL
                     LKALVRQQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVK
                     LNREDLLRKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPY
                     YVGPLARGNSRFAWMTRKSEETFTPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVL
                     PKHSLLYEYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKE
                     DYFKKIECFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLT
                     LFEDREMIEERLKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDF
                     LKSDGFANRNFMQLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQT
                     VKVVDELVKVMGRHKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKE
                     HPVENTQLQNEKLYLYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKV
                     LTRSDKNRGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKA
                     GFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQF
                     YKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIG
                     KATAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLS
                     MPQVNIVKKTEVQTGGFSKESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVV
                     AKVEKGKSKKLKSVKELLGITIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLF
                     ELENGRKRMLASAGELQKGNELALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQH
                     KHYLDEIIEQISEFSKRVILADANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGA
                     PAAFKYFDTTIDRKRYTSTKEVLDATLIHQSITGLYETRIDLSQLGGD"
     misc_feature    3139..3141
                     /gene="dcas9(D10A)"
     misc_feature    4528..4530
                     /gene="hcas9(I473F)"
     misc_feature    5629..5631
                     /gene="dcas9(H840A)"
     misc_feature    7324..7359
                     /gene="DR(CRISPR repeat)"
ORIGIN      
        1 acactgagac ttgttgagtt caaacgaaaa ttggataaag tgggatattt ttaaaatata
       61 tatttatgtt acagtaatat tgacttttaa aaaaggattg attctaatga agaaagcaga
      121 caagtaagcc tcctaaattc actttagata aaaatttagg aggcatatca aatgaacttt
      181 aataaaattg atttagacaa ttggaagaga aaagagatat ttaatcatta tttgaaccaa
      241 caaacgactt ttagtataac cacagaaatt gatattagtg ttttataccg aaacataaaa
      301 caagaaggat ataaatttta ccctgcattt attttcttag tgacaagggt gataaactca
      361 aatacagctt ttagaactgg ttacaatagc gacggagagt taggttattg ggataagtta
      421 gagccacttt atacaatttt tgatggtgta tctaaaacat tctctggtat ttggactcct
      481 gtaaagaatg acttcaaaga gttttatgat ttataccttt ctgatgtaga gaaatataat
      541 ggttcgggga aattgtttcc caaaacacct atacctgaaa atgctttttc tctttctatt
      601 attccatgga cttcatttac tgggtttaac ttaaatatca ataataatag taattacctt
      661 ctacccatta ttacagcagg aaaattcatt aataaaggta attcaatata tttaccgcta
      721 tctttacagg tacatcattc tgtttgtgat ggttatcatg caggattgtt tatgaactct
      781 attcaggaat tgtcagatag gcctaatgac tggcttttat aatatgagat aatgccgact
      841 gtacttttta cagtcggttt tctaatgtca ctaacctgcc ccgttagttg aagaaggttt
      901 ttatattaca gctccagatc catatccttc tttttctgaa ccgacttctc ctttttcgct
      961 tctttattcc aattgcttta ttgacgttga gcctcggaac ccttaacaat cccaaaactt
     1021 gtcgaatggt cggcttaata gctcacgcta tgccgacatt cgtctgcaag tttagttaag
     1081 ggttcttctc aacgcacaat aaattttctc ggcataaatg cgtggtctaa tttttatttt
     1141 taataacctt gatagcaaaa aatgccattc caatacaaaa ccacatacct ataatcgata
     1201 accacataac agtcataaag atcttgcata attcacgctg acctcccaat aactacatgg
     1261 tgttatcggg aggtcagctg ttagcactta tattttgtta ttgttcttcc tcgatttcgt
     1321 ctatcatttt gtgattaatt tctctttttt cttgttctgt taagtcataa agttcactag
     1381 ctaaatactc tttttgtttc caaatataaa aaatttgata gatatattac ggttggatca
     1441 atttctttta agtaatctaa atccccattt tttaatttct ttttagcctc tttaaataat
     1501 cctgaataaa ctaatacctg tttaccttta agtgatttat aaaatgcatc aaagactttt
     1561 tgatttattt attaaataat cactatcttt accagaatac ttagccattt catataattc
     1621 tttattatta ttttgtctta ttttttgaac ttgaacttgt gttatttctg aaatgcccgt
     1681 tacatcacgc cataaatcta accattcttg ttggctaata taatatcttt tatctgtgaa
     1741 atacgattta tttactgcaa ttaacacatg aaaatgagga ttataatcat ctcttttttt
     1801 attatatgta atctctaact tacgaacata tccctttata acactaccta ctttttttct
     1861 ctttataagt tttctaaaag aattattata acgttttatt tcattttcta attcatcact
     1921 cattacatta ggtgtagtca aagttaaaaa gataaactcc tttttctctt gctgcttaat
     1981 atattgcatc atcaaagata aacccaatgc atcttttcta gcttttctcc aagcacagac
     2041 aggacaaaat cgatttttac aagaattagc tttatataat ttctgttttt ctaaagtttt
     2101 atcagctaca aaagacagaa atgtattgca atcttcaact aaatccattt gattctctcc
     2161 aatatgacgt ttaataaatt tctgaaatac ttgatttctt tgttttttct cagtatactt
     2221 ttccatgtta taacacataa aaacaactta gttttcacaa actatgacaa taaaaaaagt
     2281 tgctttttcc cctttctatg tatgtttttt actagtcatt taaaacgata cattaatagg
     2341 tacgaaaaag caactttttt tgcgcttaaa accagtcata ccaataactt aagggtaact
     2401 agcctcgccg gcaatagtta cccttattat caagataaga aagaaaagga tttttcgcta
     2461 cgctcaaatc ctttaaaaaa acacaaaaga ccacattttt taatgtggtc tttattcttc
     2521 aactaaagca cccattagtt caacaaacgc tcgtggattc tgtgatttgg atccttccag
     2581 aagtcttttt cttgcactgt ttccttttct ttatgatagt ttacgaaatc atcctgtgga
     2641 gcttagtagg tttagcaaga tggcagcgcc taaatgtaga atgataaaag gattaagaga
     2701 ttaatttccc taaaaatgat aaaacaagcg ttttgaaagc gcttgttttt ttggtttgca
     2761 gtcagagtag aatagaagta tcaaaaaaag caccgactcg gtgccacttt ttcaagttga
     2821 taacggacta gccttatttt aacttgctat gctgttttga atggttccaa caagattatt
     2881 ttataacttt tataacaaat aatcaaggag aaattcaaag aaatttatca gccaagaatt
     2941 ttgtgttata atctatttat tattaagtat tgggtaatat tttttgaaga gatattttga
     3001 aaaagaaaaa ttaaagcata ttaaactaat ttcggaggtc attaaaacta ttattgaaat
     3061 catcaaactc attatggatt taatttaaac tttttatttt aggaggcaaa aatggataag
     3121 aaatactcaa taggcttagc tatcggcaca aatagcgtcg gatgggcggt gatcactgat
     3181 gaatataagg ttccgtctaa aaagttcaag gttctgggaa atacagaccg ccacagtatc
     3241 aaaaaaaatc ttataggggc tcttttattt gacagtggag agacagcgga agcgactcgt
     3301 ctcaaacgga cagctcgtag aaggtataca cgtcggaaga atcgtatttg ttatctacag
     3361 gagatttttt caaatgagat ggcgaaagta gatgatagtt tctttcatcg acttgaagag
     3421 tcttttttgg tggaagaaga caagaagcat gaacgtcatc ctatttttgg aaatatagta
     3481 gatgaagttg cttatcatga gaaatatcca actatctatc atctgcgaaa aaaattggta
     3541 gattctactg ataaagcgga tttgcgctta atctatttgg ccttagcgca tatgattaag
     3601 tttcgtggtc attttttgat tgagggagat ttaaatcctg ataatagtga tgtggacaaa
     3661 ctatttatcc agttggtaca aacctacaat caattatttg aagaaaaccc tattaacgca
     3721 agtggagtag atgctaaagc gattctttct gcacgattga gtaaatcaag acgattagaa
     3781 aatctcattg ctcagctccc cggtgagaag aaaaatggct tatttgggaa tctcattgct
     3841 ttgtcattgg gtttgacccc taattttaaa tcaaattttg atttggcaga agatgctaaa
     3901 ttacagcttt caaaagatac ttacgatgat gatttagata atttattggc gcaaattgga
     3961 gatcaatatg ctgatttgtt tttggcagct aagaatttat cagatgctat tttactttca
     4021 gatatcctaa gagtaaatac tgaaataact aaggctcccc tatcagcttc aatgattaaa
     4081 cgctacgatg aacatcatca agacttgact cttttaaaag ctttagttcg acaacaactt
     4141 ccagaaaagt ataaagaaat cttttttgat caatcaaaaa acggatatgc aggttatatt
     4201 gatgggggag ctagccaaga agaattttat aaatttatca aaccaatttt agaaaaaatg
     4261 gatggtactg aggaattatt ggtgaaacta aatcgtgaag atttgctgcg caagcaacgg
     4321 acctttgaca acggctctat tccccatcaa attcacttgg gtgagctgca tgctattttg
     4381 agaagacaag aagactttta tccattttta aaagacaatc gtgagaagat tgaaaaaatc
     4441 ttgacttttc gaattcctta ttatgttggt ccattggcgc gtggcaatag tcgttttgca
     4501 tggatgactc ggaagtctga agaaacattt accccatgga attttgaaga agttgtcgat
     4561 aaaggtgctt cagctcaatc atttattgaa cgcatgacaa actttgataa aaatcttcca
     4621 aatgaaaaag tactaccaaa acatagtttg ctttatgagt attttacggt ttataacgaa
     4681 ttgacaaagg tcaaatatgt tactgaagga atgcgaaaac cagcatttct ttcaggtgaa
     4741 cagaagaaag ccattgttga tttactcttc aaaacaaatc gaaaagtaac cgttaagcaa
     4801 ttaaaagaag attatttcaa aaaaatagaa tgttttgata gtgttgaaat ttcaggagtt
     4861 gaagatagat ttaatgcttc attaggtacc taccatgatt tgctaaaaat tattaaagat
     4921 aaagattttt tggataatga agaaaatgaa gatatcttag aggatattgt tttaacattg
     4981 accttatttg aagataggga gatgattgag gaaagactta aaacatatgc tcacctcttt
     5041 gatgataagg tgatgaaaca gcttaaacgt cgccgttata ctggttgggg acgtttgtct
     5101 cgaaaattga ttaatggtat tagggataag caatctggca aaacaatatt agattttttg
     5161 aaatcagatg gttttgccaa tcgcaatttt atgcagctga tccatgatga tagtttgaca
     5221 tttaaagaag acattcaaaa agcacaagtg tctggacaag gcgatagttt acatgaacat
     5281 attgcaaatt tagctggtag ccctgctatt aaaaaaggta ttttacagac tgtaaaagtt
     5341 gttgatgaat tggtcaaagt aatggggcgg cataagccag aaaatatcgt tattgaaatg
     5401 gcacgtgaaa atcagacaac tcaaaagggc cagaaaaatt cgcgagagcg tatgaaacga
     5461 atcgaagaag gtatcaaaga attaggaagt cagattctta aagagcatcc tgttgaaaat
     5521 actcaattgc aaaatgaaaa gctctatctc tattatctcc aaaatggaag agacatgtat
     5581 gtggaccaag aattagatat taatcgttta agtgattatg atgtcgatgc cattgttcca
     5641 caaagtttcc ttaaagacga ttcaatagac aataaggtct taacgcgttc tgataaaaat
     5701 cgtggtaaat cggataacgt tccaagtgaa gaagtagtca aaaagatgaa aaactattgg
     5761 agacaacttc taaacgccaa gttaatcact caacgtaagt ttgataattt aacgaaagct
     5821 gaacgtggag gtttgagtga acttgataaa gctggtttta tcaaacgcca attggttgaa
     5881 actcgccaaa tcactaagca tgtggcacaa attttggata gtcgcatgaa tactaaatac
     5941 gatgaaaatg ataaacttat tcgagaggtt aaagtgatta ccttaaaatc taaattagtt
     6001 tctgacttcc gaaaagattt ccaattctat aaagtacgtg agattaacaa ttaccatcat
     6061 gcccatgatg cgtatctaaa tgccgtcgtt ggaactgctt tgattaagaa atatccaaaa
     6121 cttgaatcgg agtttgtcta tggtgattat aaagtttatg atgttcgtaa aatgattgct
     6181 aagtctgagc aagaaatagg caaagcaacc gcaaaatatt tcttttactc taatatcatg
     6241 aacttcttca aaacagaaat tacacttgca aatggagaga ttcgcaaacg ccctctaatc
     6301 gaaactaatg gggaaactgg agaaattgtc tgggataaag ggcgagattt tgccacagtg
     6361 cgcaaagtat tgtccatgcc ccaagtcaat attgtcaaga aaacagaagt acagacaggc
     6421 ggattctcca aggagtcaat tttaccaaaa agaaattcgg acaagcttat tgctcgtaaa
     6481 aaagactggg atccaaaaaa atatggtggt tttgatagtc caacggtagc ttattcagtc
     6541 ctagtggttg ctaaggtgga aaaagggaaa tcgaagaagt taaaatccgt taaagagtta
     6601 ctagggatca caattatgga aagaagttcc tttgaaaaaa atccgattga ctttttagaa
     6661 gctaaaggat ataaggaagt taaaaaagac ttaatcatta aactacctaa atatagtctt
     6721 tttgagttag aaaacggtcg taaacggatg ctggctagtg ccggagaatt acaaaaagga
     6781 aatgagctgg ctctgccaag caaatatgtg aattttttat atttagctag tcattatgaa
     6841 aagttgaagg gtagtccaga agataacgaa caaaaacaat tgtttgtgga gcagcataag
     6901 cattatttag atgagattat tgagcaaatc agtgaatttt ctaagcgtgt tattttagca
     6961 gatgccaatt tagataaagt tcttagtgca tataacaaac atagagacaa accaatacgt
     7021 gaacaagcag aaaatattat tcatttattt acgttgacga atcttggagc tcccgctgct
     7081 tttaaatatt ttgatacaac aattgatcgt aaacgatata cgtctacaaa agaagtttta
     7141 gatgccactc ttatccatca atccatcact ggtctttatg aaacacgcat tgatttgagt
     7201 cagctaggag gtgactgata atactcttaa taaatgcagt aatacagggg cttttcaaga
     7261 ctgaagtcta gctgagacaa atagtgcgat tacaaaattt tttagacaaa aatagtctac
     7321 gaggttttag agctatgctg ttttgaatgg tcccaaaaca acattgccga tgataacttg
     7381 agaaagaggg ttaataccag cagtcggata ccttcctatt ctttctgtta aagcgttttc
     7441 atgttataat aggcaaaaga agagtagtgt gatggaacaa acatttttta tgattaagcc
     7501 atatggggtt aagcaagggg aggtagttgg agaggtttta cggtggattg aacgcctaag
     7561 atttacgttt aagcgattcg agctaagaca agctagttcg aaatacttgg ctaagcacga
     7621 cgaggccttg gtgataaacc ttttgatcct aaacttaaag cttacatgac aagtggtcct
     7681 gttttaattg ggataattct tggggactaa
//
