LOCUS       pWJ418                  8245 bp    DNA     circular SYN 30-NOV-2016
DEFINITION  
ACCESSION   pWJ418
KEYWORDS    .
SOURCE      Unknown.
  ORGANISM  Unknown
            Unclassified.
REFERENCE   1  (bases 1 to 8245)
  AUTHORS   Self
  JOURNAL   Unpublished.
COMMENT     SECID/File created by Clone Manager, Scientific & Educational Software
FEATURES             Location/Qualifiers
     misc_signal     514..519
     misc_feature    514..569
                     /gene="pSpac"
     misc_signal     537..542
     misc_feature    549..554
                     /gene="lacO"
     misc_feature    564..569
                     /gene="lacO"
     CDS             601..1470
                     /gene="cas1"
     CDS             1467..1808
                     /gene="cas2"
     CDS             1798..2460
                     /gene="csn2"
     CDS             2618..3859
                     /gene="pre"
     CDS             4386..5330
                     /gene="repC"
     CDS             5492..6871
                     /gene="tet"
     CDS             7139..8218
                     /gene="lacI"
ORIGIN      
        1 ggatcccggg aggaaataat tctatgagtc gcttttgtaa atttggaaag ttacacgtta
       61 ctaaagggaa tgtagataaa ttattaggta tactactgac agcttccaag gagctaaaga
      121 ggtccctaga ctctagaccc ggggatctct gcagtcgcga tgattaatta attcagaacg
      181 ctcggttgcc gccgggcgtt ttttatgcag caatggcaag aacgttgctc tagaataatt
      241 ctacacagcc cagtccagac tattcggcac tgaaattatg ggaattctac acagcccagt
      301 ccagactatt cggcactgaa attatgggtg aagtggtcaa gacctcacta ggcaccttaa
      361 aaatagcgca ccctgaagaa gatttatttg aggtagccct tgcctaccta gcttccaaga
      421 aagatatcct aacagcacaa gagcggaaag atgttttgtt ctacatccag aacaacctct
      481 gctaaaattc ctgaaaaatt ttgcaaaaag ttgttgactt tatctacaag gtgtggcata
      541 atgtgtggaa ttgtgagcgg ataacaatta agcttatttg agtcagctag gaggtgactg
      601 atggctggtt ggcgtactgt tgtggtaaat acccactcga aattatccta taagaataat
      661 catctgattt ttaaggatgc ctataaaacg gagctgatcc atttatcaga aattgatatt
      721 ttgttattag aaacgaccga tattgtcttg tccactatgc tggtaaaacg gctagtggat
      781 gagaatgtcc ttgtcatatt ctgtgatgat aaacgattac caacagctat gctgatgcct
      841 ttttatggtc gtcatgattc gagtttacag cttgggaaac aaatgtcctg gtcagaaaca
      901 gtcaaatcgc aggtttggac gacgattatt gctcaaaaga ttttgaatca atcttgctat
      961 ctaggagcat gctcctattt tgaaaaatcc caatctatta tggatttata tcatggtttg
     1021 gaaaattttg atccgagtaa tcgagaaggg catgcagcga gaatttattt taatacactt
     1081 tttgggaacg atttctcaag agatttggag catccaatca atgcaggtct ggattatggt
     1141 tatactttat tattgagtat gtttgcgcgt gaagtggttg tgtctggatg tatgactcag
     1201 tttgggctta aacacgctaa tcagtttaat cagttcaatt ttgctagcga tattatggaa
     1261 ccatttaggc ctttagtgga taagattgtt tatgaaaatc gaaatcagcc ttttcccaaa
     1321 ataaagagag agttatttac tttgttttca gatacatttt catataatgg taaagagatg
     1381 tatctcacga atattattag cgattatact aaaaaagttg tcaaagctct gaataatgaa
     1441 gggaaaggag ttcctgaatt taggatatga gttatagata tatgagaatg atacttatgt
     1501 ttgatatgcc gacggacacc gctgaggaac gaaaagccta tcgaaaattt cggaaatttt
     1561 tacttagtga agggtttatc atgcatcaat tttctattta tagtaagttg ctgttgaata
     1621 atacagctaa caatgccatg attggtcggc tgagggagca taatcctaat aaaggaaata
     1681 ttacattact aacggtcacg gaaaaacagt ttgcacgaat gatttattta catggtgaaa
     1741 gaaataattg tattgcaaac tccgatgaaa gacttgtatt tcttggggag gcttttgatg
     1801 aatcttaatt tttccttact agatgaaccg attccattaa gaggcggtac aattcttgtg
     1861 ctcgaagatg tctgtgtatt ttcaaaaata gtgcaatatt gttaccaata tgaggaagat
     1921 tctgaactta aattttttga tcacaagatg aaaacaatca aagaatcaga aatcatgctt
     1981 gtaacagata ttttaggatt tgatgttaac tcctcaacca ttttaaaatt gattcatgca
     2041 gatttagaat ctcaatttaa tgagaaaccc gaagtgaaat cgatgattga caaattggtt
     2101 gctacgatta cagaactgat tgtctttgaa tgcttagaaa atgaattaga tttagagtat
     2161 gatgaaatca caatcctgga attgattaag tccttaggag taaaagtaga aacgcaaagt
     2221 gatactattt ttgaaaaatg tctagagata cttcaaattt tcaaatatct cactaagaaa
     2281 aagttgctta tttttgtcaa tagcggagct tttctaacaa aggatgaagt ggctagttta
     2341 caagagtata tatcattgac aaatttaaca gttctctttt tagaaccacg tgaactatat
     2401 gattttccgc agtatatttt agatgaagat tatttcttaa taactaaaaa tatggtataa
     2461 tactcttaat aaatgcagta atacaggggc ttttcgcaaa aacaggttta agcctcgcag
     2521 agcacacgta ttaacgactt attaaaaata agtctagtgt gttagactta aactattaaa
     2581 tacacataaa acctttgtgc ttaggagtga tttttatatg tcttattcca ttgttagagt
     2641 ttcaaaagtt aaatctggaa caaatacaac gggcatacaa aaacatgttc aaagagaaaa
     2701 taataattat gaaaatgaag atatagacca tagtaaaact tacttaaatt atgatttggt
     2761 aaatgctaat aaacagaatt ttaataactt gattgatgaa aaaatcgaac agaattatac
     2821 aggcaaaaga aaaattagaa cagacgcgat taaacacatt gatggtttaa ttacatcaga
     2881 caatgatttc tttgataatc aaacgccaga agatacaaag cagttttttg aatatgctaa
     2941 agagttttta gaacaagaat acggtaaaga taatttatta tatgcaacag ttcacatgga
     3001 cgaaaaaaca ccacatatgc attatggcgt tgttccaata actgatgatg gtcgtttaag
     3061 tgctaaagaa gttgtaggta ataaaaaagc tttaacagcg tttcaagata gatttaatga
     3121 gcatgttaaa caacgaggat atggtttaga acgtgggcaa tcaagacaag taacaaatgc
     3181 taaacatgag caaataagtc agtataaaca aaaaacagaa tatcataagc aagaatatga
     3241 acgtgagagc caaaaaacag accatataaa gcaaaagaac gataaattaa tgcaagagta
     3301 ccaaaaatcg ttaaatacgc ttaaaaagcc tataaatgtt ccgtatgagc aagaaactga
     3361 aaaagtaggt ggtttattta gcaaagagat acaagaagct ggaaatgttg taataagcca
     3421 aaaagatttc aatgaatttc agaaacagat aaaagctgct caagatattt cggaagatta
     3481 cgagtatata aagtctggta gagccttaga tgataaagat aaggaaatac gagagaaaga
     3541 tgatttatta aataaagcag ttgagcgtat tgaaaacgca gacgataatt ttaaccaact
     3601 ttacgaaaat gcaaagccac ttaaagagaa tatagaaata gcgttaaagc ttttaaaaat
     3661 cttactaaaa gagttagaac gagttttagg aagaaatacc tttgcggaaa gagttaataa
     3721 gttaacagaa gatgaaccaa aactaaatgg tttagcagga aacttagata aaaaaatgaa
     3781 tccagaatta tattcagaac aggaacagca acaagaacaa caaaagaatc aaaaacgaga
     3841 tagaggtatg cacttataga acatgcattt atgccgagaa aacttattgg ttggaatggg
     3901 ctatgtgtta gctaacttgt tagcgagttg gttggacttg aattgggatt aatcccaaga
     3961 aagtaccaac tcaacaacac ataaagccct gtaggttccg accaataagg aaattggaat
     4021 aaagcaataa aaggagttga agaaatgaaa ttcagagaag cctttgagaa ttttataaca
     4081 agtaagtatg tacttggtgt tttagtagtt ttaactgttt accagataat acaaatgctt
     4141 aaataaaaaa agacttgatc tgattagacc aaatcttttg atagtgttat attaataaca
     4201 aaataaaaag gagtcgctca cgccctgacc aaagtttgtg aacgacatca ttcaaagaaa
     4261 aaaacactga gttgttttta taatcttgta tatttagata ttaaacgata tttaaatata
     4321 catcaagata tatatttggg tgagcgattc cttaaacgaa attgagatta aggagtcgat
     4381 tttttatgta taaaaacaat catgcaaatc attcaaatca tttggaaaat cacgatttag
     4441 acaatttttc taaaaccggc tactctaata gccggttgga cgcacatact gtgtgcatat
     4501 ctgatccaaa attaagtttt gatgcaatga cgatcgttgg aaatctcaac cgagacaacg
     4561 ctcaagccct ttctaaattt atgagtgtag agccccaaat aagactttgg gatattcttc
     4621 aaacaaagtt taaagctaaa gcacttcaag aaaaagttta tattgaatat gacaaagtga
     4681 aagcagatag ttgggataga cgtaatatgc gtattgaatt taatccaaac aaacttacac
     4741 gagatgaaat gatttggtta aaacaaaata taataagcta catggaagat gacggtttta
     4801 caagattaga tttagccttt gattttgaag aagatttgag tgactactat gcaatgtctg
     4861 ataaagcagt taagaaaact attttttatg gtcgtaatgg taagccagaa acaaaatatt
     4921 ttggcgtgag agatagtaat agatttatta gaatttataa taaaaagcaa gaacgtaaag
     4981 ataatgcaga tgctgaagtt atgtctgaac atttatggcg tgtagaaatc gaacttaaaa
     5041 gagatatggt ggattactgg aatgattgct ttagtgattt acatatcttg caaccagatt
     5101 ggaaaactat ccaacgcact gcggatagag caatagtttt tatgttattg agtgatgaag
     5161 aagaatgggg aaagcttcac agaaattcta gaacaaaata taagaatttg ataaaagaaa
     5221 tttcgccagt cgatttaacg gacttaatga aatcgacttt aaaagcgaac gaaaaacaat
     5281 tgcaaaaaca aatcgatttt tggcaacatg aatttaaatt ttggaaatag tgtacatatt
     5341 aatattactg aacaaaaatg atatatttaa actattctaa tttaggagga tttttttatg
     5401 aagtgtctat ttaaaaattt ggggaattta tatgaggtga aagaataatt tacccctata
     5461 aactttagtc acctcaagta aagaggtaaa attgtttagt ttatataaaa aatttaaagg
     5521 tttgttttat agcgttttat tttggctttg tattctttca ttttttagtg tattaaatga
     5581 aatggtttta aatgtttctt tacctgatat tgcaaatcat tttaatacta ctcctggaat
     5641 tacaaactgg gtaaacactg catatatgtt aactttttcg ataggaacag cagtatatgg
     5701 aaaattatct gattatataa atataaaaaa attgttaatt attggtatta gtttgagctg
     5761 tcttggttca ttgattgctt ttattggtca caatcacttt tttattttga tttttggtag
     5821 gttagtacaa ggagtaggat ctgctgcatt cccttcactg attatggtgg ttgtagctag
     5881 aaatattaca agaaaaaaac aaggcaaagc ctttggtttt ataggatcaa ttgtagcttt
     5941 aggtgaaggg ttaggtcctt caataggggg aataatagca cattatattc attggtctta
     6001 cctacttata cttcctatga ttacaatagt aactatacct tttcttatta aagtaatggt
     6061 acctggtaaa tcaacaaaaa atacattaga tatcgtaggt attgttttaa tgtctataag
     6121 tattatatgt tttatgttat ttacgacaaa ttataattgg acttttttaa tactcttcac
     6181 aatctttttt gtgattttta ttaaacatat ttcaagagtt tctaaccctt ttattaatcc
     6241 taaactaggg aaaaacattc cgtttatgct tggtttgttt tctggtgggc taatattttc
     6301 tatagtagct ggttttatat caatggtgcc ttatatgatg aaaactattt atcatgtaaa
     6361 tgtagcgaca ataggtaata gtgttatttt tcctggaacc atgagtgtta ttgtttttgg
     6421 ttattttggt ggttttttag tggatagaaa aggatcatta tttgttttta ttttaggatc
     6481 attgtctatc tctataagtt ttttaactat tgcatttttt gttgagttta gtatgtggtt
     6541 gactactttt atgtttatat ttgttatggg cggattatct tttactaaaa cagttatatc
     6601 aaaaatagta tcaagtagtc tttctgaaga agaagttgct tctggaatga gtttgctaaa
     6661 tttcacaagt tttttatcag agggaacagg tatagcaatt gtaggaggtt tattgtcact
     6721 acaattgatt aatcgtaaac tagttctgga atttataaat tattcttctg gagtgtatag
     6781 taatattctt gtagccatgg ctatccttat tattttatgt tgtcttttga cgattattgt
     6841 atttaaacgt tctgaaaagc agtttgaata gttatattat attttggttt agaactatga
     6901 gtggctagtg gaaacgaggt catcatttcc ttccgaaaaa acggttgcat ttaaatctta
     6961 catatgtaat actttcaaag actacatttg taagatttga tgtttgagtc ggctgaaaga
     7021 tcgtacgtac caattattgt ttcgtgattg ttcaagccat aacactgtag ggatagtgga
     7081 aagagtgctt catctggtta cgatcaatca aatattcaaa cggagggaga cgattttgat
     7141 gaaaccagta acgttatacg atgtcgcaga gtatgccggt gtctcttatc agaccgtttc
     7201 ccgcgtggtg aaccaggcca gccacgtttc tgcgaaaacg cgggaaaaag tggaagcggc
     7261 gatggcggag ctgaattaca ttcccaaccg cgtggcacaa caactggcgg gcaaacagtc
     7321 gttgctgatt ggcgttgcca cctccagtct ggccctgcac gcgccgtcgc aaattgtcgc
     7381 ggcgattaaa tctcgcgccg atcaactggg tgccagcgtg gtggtgtcga tggtagaacg
     7441 aagcggcgtc gaagcctgta aagcggcggt gcacaatctt ctcgcgcaac gcgtcagtgg
     7501 gctgatcatt aactatccgc tggatgacca ggatgccatt gctgtggaag ctgcctgcac
     7561 taatgttccg gcgttatttc ttgatgtctc tgaccagaca cccatcaaca gtattatttt
     7621 ctcccatgaa gacggtacgc gactgggcgt ggagcatctg gtcgcattgg gtcaccagca
     7681 aatcgcgctg ttagcgggcc cattaagttc tgtctcggcg cgtctgcgtc tggctggctg
     7741 gcataaatat ctcactcgca atcaaattca gccgatagcg gaacgggaag gcgactggag
     7801 tgccatgtcc ggttttcaac aaaccatgca aatgctgaat gagggcatcg ttcccactgc
     7861 gatgctggtt gccaacgatc agatggcgct gggcgcaatg cgcgccatta ccgagtccgg
     7921 gctgcgcgtt ggtgcggata tctcggtagt gggatacgac gataccgaag acagctcatg
     7981 ttatatcccg ccgtcaacca ccatcaaaca ggattttcgc ctgctggggc aaaccagcgt
     8041 ggaccgcttg ctgcaactct ctcagggcca ggcggtgaag ggcaatcagc tgttgcccgt
     8101 ctcactggtg aaaagaaaaa ccaccctggc gcccaatacg caaaccgcct ctccccgcgc
     8161 gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg
     8221 agcgcaacgc aattaatgtg agtta
//
