LOCUS       POPL118_pSH100_M        6603 bp ds-DNA     circular     11-APR-2016
DEFINITION  .
ACCESSION   
VERSION     
SOURCE      .
  ORGANISM  .
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     misc_feature    421..423
                     /label=Start Codon
                     /ApEinfo_fwdcolor=#ff0000
                     /ApEinfo_revcolor=#ff0000
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    385..414
                     /label=MET25 promoter?
                     /ApEinfo_fwdcolor=#ff8080
                     /ApEinfo_revcolor=#ff8080
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1589..1828
                     /label=CYC1 terminator
                     /ApEinfo_fwdcolor=#c0c0c0
                     /ApEinfo_revcolor=#c0c0c0
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    4557..5417
                     /label=AmpR
                     /ApEinfo_fwdcolor=#ffff80
                     /ApEinfo_revcolor=#ffff80
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    5572..6191
                     /label=pBR322Ori
                     /ApEinfo_fwdcolor=#808000
                     /ApEinfo_revcolor=#808000
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1558..1560
                     /label=Stop Codon
                     /ApEinfo_fwdcolor=#ff0000
                     /ApEinfo_revcolor=#ff0000
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    811..1515
                     /label=mCherry
                     /ApEinfo_fwdcolor=#ff8000
                     /ApEinfo_revcolor=#ff8000
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    join(436..692,694..786)
                     /label=MS2 coat protein
                     /ApEinfo_fwdcolor=#ffff00
                     /ApEinfo_revcolor=#ffff00
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    2626..3426
                     /label=New Feature
                     /ApEinfo_fwdcolor=#008080
                     /ApEinfo_revcolor=#008080
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1534..1557
                     /label=FLAG
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=cyan
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    30..384
                     /label=enhancer
                     /ApEinfo_fwdcolor=#e8e8e8
                     /ApEinfo_revcolor=#e8e8e8
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
ORIGIN
        1 ACCCTCACTA AAGGGAACAA AAGCTGGAGC TCCGGATGCA AGGGTTCGAA TCCCTTAGCT
       61 CTCATTATTT TTTGCTTTTT CTCTTGAGGT CACATGATCG CAAAATGGCA AATGGCACGT
      121 GAAGCTGTCG ATATTGGGGA ACTGTGGTGG TTGGCAAATG ACTAATTAAG TTAGTCAAGG
      181 CGCCATCCTC ATGAAAACTG TGTAACATAA TAACCGAAGT GTCGAAAAGG TGGCACCTTG
      241 TCCAATTGAA CACGCTCGAT GAAAAAAATA AGATATATAT AAGGTTAAGT AAAGCGTCTG
      301 TTAGAAAGGA AGTTTTTCCT TTTTCTTGCT CTCTTGTCTT TTCATCTACT ATTTCCTTCG
      361 TGTAATACAG GGTCGTCAGA TACATAGATA CAATTCTATT ACCCCCATCC ATACTCTAGA
      421 ATGCTAGCCG TTAAAATGGC TTCTAACTTT ACTCAGTTCG TTCTCGTCGA CAATGGCGGA
      481 ACTGGCGACG TGACTGTCGC CCCAAGCAAC TTCGCTAACG GGATCGCTGA ATGGATCAGC
      541 TCTAACTCGC GTTCACAGGC TTACAAAGTA ACCTGTAGCG TTCGTCAGAG CTCTGCGCAG
      601 AATCGCAAAT ACACCATCAA AGTCGAGGTG CCTAAAGGCG CCTGGCGTTC GTACTTAAAT
      661 ATGGAACTAA CCATTCCAAT TTTCGCCACG AATTCCGACT GCGAGCTTAT TGTTAAGGCA
      721 ATGCAAGGTC TCCTAAAAGA TGGAAACCCG ATTCCCTCAG CAATCGCAGC AAACTCCGGC
      781 ATCTACGCGG ATCCACCGGT CGCCACCATG GTGAGCAAGG GCGAGGAGGA TAACATGGCC
      841 ATCATCAAGG AGTTCATGCG CTTCAAGGTG CACATGGAGG GCTCCGTGAA CGGCCACGAG
      901 TTCGAGATCG AGGGCGAGGG CGAGGGCCGC CCCTACGAGG GCACCCAGAC CGCCAAGCTG
      961 AAGGTGACCA AGGGTGGCCC CCTGCCCTTC GCCTGGGACA TCCTGTCCCC TCAGTTCATG
     1021 TACGGCTCCA AGGCCTACGT GAAGCACCCC GCCGACATCC CCGACTACTT GAAGCTGTCC
     1081 TTCCCCGAGG GCTTCAAGTG GGAGCGCGTG ATGAACTTCG AGGACGGCGG CGTGGTGACC
     1141 GTGACCCAGG ACTCCTCCCT GCAGGACGGC GAGTTCATCT ACAAGGTGAA GCTGCGCGGC
     1201 ACCAACTTCC CCTCCGACGG CCCCGTAATG CAGAAGAAGA CCATGGGCTG GGAGGCCTCC
     1261 TCCGAGCGGA TGTACCCCGA GGACGGCGCC CTGAAGGGCG AGATCAAGCA GAGGCTGAAG
     1321 CTGAAGGACG GCGGCCACTA CGACGCTGAG GTCAAGACCA CCTACAAGGC CAAGAAGCCC
     1381 GTGCAGCTGC CCGGCGCCTA CAACGTCAAC ATCAAGTTGG ACATCACCTC CCACAACGAG
     1441 GACTACACCA TCGTGGAACA GTACGAACGC GCCGAGGGCC GCCACTCCAC CGGCGGCATG
     1501 GACGAGCTGT ACAAGTCAAG AGGGCCCTTC GAAGATTATA AAGACGATGA CGATAAGTAA
     1561 ATCGATACCG TCGACCTCGA GTCATGTAAT TAGTTATGTC ACGCTTACAT TCACGCCCTC
     1621 CCCCCACATC CGCTCTAACC GAAAAGGAAG GAGTTAGACA ACCTGAAGTC TAGGTCCCTA
     1681 TTTATTTTTT TATAGTTATG TTAGTATTAA GAACGTTATT TATATTTCAA ATTTTTCTTT
     1741 TTTTTCTGTA CAGACGCGTG TACGCATGTA ACATTATACT GAAAACCTTG CTTGAGAAGG
     1801 TTTTGGGACG CTCGAAGGCT TTAATTTGCG GCCGGTACCC AATTCGCCCT ATAGTGAGTC
     1861 GTATTACGCG CGCTCACTGG CCGTCGTTTT ACAACGTCGT GACTGGGAAA ACCCTGGCGT
     1921 TACCCAACTT AATCGCCTTG CAGCACATCC CCCTTTCGCC AGCTGGCGTA ATAGCGAAGA
     1981 GGCCCGCACC GATCGCCCTT CCCAACAGTT GCGCAGCCTG AATGGCGAAT GGCGCGACGC
     2041 GCCCTGTAGC GGCGCATTAA GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG TGACCGCTAC
     2101 ACTTGCCAGC GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC TCGCCACGTT
     2161 CGCCGGCTTT CCCCGTCAAG CTCTAAATCG GGGGCTCCCT TTAGGGTTCC GATTTAGTGC
     2221 TTTACGGCAC CTCGACCCCA AAAAACTTGA TTAGGGTGAT GGTTCACGTA GTGGGCCATC
     2281 GCCCTGATAG ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA ATAGTGGACT
     2341 CTTGTTCCAA ACTGGAACAA CACTCAACCC TATCTCGGTC TATTCTTTTG ATTTATAAGG
     2401 GATTTTGCCG ATTTCGGCCT ATTGGTTAAA AAATGAGCTG ATTTAACAAA AATTTAACGC
     2461 GAATTTTAAC AAAATATTAA CGTTTACAAT TTCCTGATGC GGTATTTTCT CCTTACGCAT
     2521 CTGTGCGGTA TTTCACACCG CATAGGGTAA TAACTGATAT AATTAAATTG AAGCTCTAAT
     2581 TTGTGAGTTT AGTATACATG CATTTACTTA TAATACAGTT TTTTAGTTTT GCTGGCCGCA
     2641 TCTTCTCAAA TATGCTTCCC AGCCTGCTTT TCTGTAACGT TCACCCTCTA CCTTAGCATC
     2701 CCTTCCCTTT GCAAATAGTC CTCTTCCAAC AATAATAATG TCAGATCCTG TAGAGACCAC
     2761 ATCATCCACG GTTCTATACT GTTGACCCAA TGCGTCTCCC TTGTCATCTA AACCCACACC
     2821 GGGTGTCATA ATCAACCAAT CGTAACCTTC ATCTCTTCCA CCCATGTCTC TTTGAGCAAT
     2881 AAAGCCGATA ACAAAATCTT TGTCGCTCTT CGCAATGTCA ACAGTACCCT TAGTATATTC
     2941 TCCAGTAGAT AGGGAGCCCT TGCATGACAA TTCTGCTAAC ATCAAAAGGC CTCTAGGTTC
     3001 CTTTGTTACT TCTTCTGCCG CCTGCTTCAA ACCGCTAACA ATACCTGGGC CCACCACACC
     3061 GTGTGCATTC GTAATGTCTG CCCATTCTGC TATTCTGTAT ACACCCGCAG AGTACTGCAA
     3121 TTTGACTGTA TTACCAATGT CAGCAAATTT TCTGTCTTCG AAGAGTAAAA AATTGTACTT
     3181 GGCGGATAAT GCCTTTAGCG GCTTAACTGT GCCCTCCATG GAAAAATCAG TCAAGATATC
     3241 CACATGTGTT TTTAGTAAAC AAATTTTGGG ACCTAATGCT TCAACTAACT CCAGTAATTC
     3301 CTTGGTGGTA CGAACATCCA ATGAAGCACA CAAGTTTGTT TGCTTTTCGT GCATGATATT
     3361 AAATAGCTTG GCAGCAACAG GACTAGGATG AGTAGCAGCA CGTTCCTTAT ATGTAGCTTT
     3421 CGACATGATT TATCTTCGTT TCCTGCAGGT TTTTGTTCTG TGCAGTTGGG TTAAGAATAC
     3481 TGGGCAATTT CATGTTTCTT CAACACTACA TATGCGTATA TATACCAATC TAAGTCTGTG
     3541 CTCCTTCCTT CGTTCTTCCT TCTGTTCGGA GATTACCGAA TCAAAAAAAT TTCAAAGAAA
     3601 CCGAAATCAA AAAAAAGAAT AAAAAAAAAA TGATGAATTG AATTGAAAAG CTGTGGTATG
     3661 GTGCACTCTC AGTACAATCT GCTCTGATGC CGCATAGTTA AGCCAGCCCC GACACCCGCC
     3721 AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC
     3781 TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC
     3841 GAGACGAAAG GGCCTCGTGA TACGCCTATT TTTATAGGTT AATGTCATGA TAATAATGGT
     3901 TTCTTAGGAC GGATCGCTTG CCTGTAACTT ACACGCGCCT CGTATCTTTT AATGATGGAA
     3961 TAATTTGGGA ATTTACTCTG TGTTTATTTA TTTTTATGTT TTGTATTTGG ATTTTAGAAA
     4021 GTAAATAAAG AAGGTAGAAG AGTTACGGAA TGAAGAAAAA AAAATAAACA AAGGTTTAAA
     4081 AAATTTCAAC AAAAAGCGTA CTTTACATAT ATATTTATTA GACAAGAAAA GCAGATTAAA
     4141 TAGATATACA TTCGATTAAC GATAAGTAAA ATGTAAAATC ACAGGATTTT CGTGTGTGGT
     4201 CTTCTACACA GACAAGATGA AACAATTCGG CATTAATACC TGAGAGCAGG AAGAGCAAGA
     4261 TAAAAGGTAG TATTTGTTGG CGATCCCCCT AGAGTCTTTT ACATCTTCGG AAAACAAAAA
     4321 CTATTTTTTC TTTAATTTCT TTTTTTACTT TCTATTTTTA ATTTATATAT TTATATTAAA
     4381 AAATTTAAAT TATAATTATT TTTATAGCAC GTGATGAAAA GGACCCAGGT GGCACTTTTC
     4441 GGGGAAATGT GCGCGGAACC CCTATTTGTT TATTTTTCTA AATACATTCA AATATGTATC
     4501 CGCTCATGAG ACAATAACCC TGATAAATGC TTCAATAATA TTGAAAAAGG AAGAGTATGA
     4561 GTATTCAACA TTTCCGTGTC GCCCTTATTC CCTTTTTTGC GGCATTTTGC CTTCCTGTTT
     4621 TTGCTCACCC AGAAACGCTG GTGAAAGTAA AAGATGCTGA AGATCAGTTG GGTGCACGAG
     4681 TGGGTTACAT CGAACTGGAT CTCAACAGCG GTAAGATCCT TGAGAGTTTT CGCCCCGAAG
     4741 AACGTTTTCC AATGATGAGC ACTTTTAAAG TTCTGCTATG TGGCGCGGTA TTATCCCGTA
     4801 TTGACGCCGG GCAAGAGCAA CTCGGTCGCC GCATACACTA TTCTCAGAAT GACTTGGTTG
     4861 AGTACTCACC AGTCACAGAA AAGCATCTTA CGGATGGCAT GACAGTAAGA GAATTATGCA
     4921 GTGCTGCCAT AACCATGAGT GATAACACTG CGGCCAACTT ACTTCTGACA ACGATCGGAG
     4981 GACCGAAGGA GCTAACCGCT TTTTTGCACA ACATGGGGGA TCATGTAACT CGCCTTGATC
     5041 GTTGGGAACC GGAGCTGAAT GAAGCCATAC CAAACGACGA GCGTGACACC ACGATGCCTG
     5101 TAGCAATGGC AACAACGTTG CGCAAACTAT TAACTGGCGA ACTACTTACT CTAGCTTCCC
     5161 GGCAACAATT AATAGACTGG ATGGAGGCGG ATAAAGTTGC AGGACCACTT CTGCGCTCGG
     5221 CCCTTCCGGC TGGCTGGTTT ATTGCTGATA AATCTGGAGC CGGTGAGCGT GGGTCTCGCG
     5281 GTATCATTGC AGCACTGGGG CCAGATGGTA AGCCCTCCCG TATCGTAGTT ATCTACACGA
     5341 CGGGGAGTCA GGCAACTATG GATGAACGAA ATAGACAGAT CGCTGAGATA GGTGCCTCAC
     5401 TGATTAAGCA TTGGTAACTG TCAGACCAAG TTTACTCATA TATACTTTAG ATTGATTTAA
     5461 AACTTCATTT TTAATTTAAA AGGATCTAGG TGAAGATCCT TTTTGATAAT CTCATGACCA
     5521 AAATCCCTTA ACGTGAGTTT TCGTTCCACT GAGCGTCAGA CCCCGTAGAA AAGATCAAAG
     5581 GATCTTCTTG AGATCCTTTT TTTCTGCGCG TAATCTGCTG CTTGCAAACA AAAAAACCAC
     5641 CGCTACCAGC GGTGGTTTGT TTGCCGGATC AAGAGCTACC AACTCTTTTT CCGAAGGTAA
     5701 CTGGCTTCAG CAGAGCGCAG ATACCAAATA CTGTCCTTCT AGTGTAGCCG TAGTTAGGCC
     5761 ACCACTTCAA GAACTCTGTA GCACCGCCTA CATACCTCGC TCTGCTAATC CTGTTACCAG
     5821 TGGCTGCTGC CAGTGGCGAT AAGTCGTGTC TTACCGGGTT GGACTCAAGA CGATAGTTAC
     5881 CGGATAAGGC GCAGCGGTCG GGCTGAACGG GGGGTTCGTG CACACAGCCC AGCTTGGAGC
     5941 GAACGACCTA CACCGAACTG AGATACCTAC AGCGTGAGCT ATGAGAAAGC GCCACGCTTC
     6001 CCGAAGGGAG AAAGGCGGAC AGGTATCCGG TAAGCGGCAG GGTCGGAACA GGAGAGCGCA
     6061 CGAGGGAGCT TCCAGGGGGA AACGCCTGGT ATCTTTATAG TCCTGTCGGG TTTCGCCACC
     6121 TCTGACTTGA GCGTCGATTT TTGTGATGCT CGTCAGGGGG GCGGAGCCTA TGGAAAAACG
     6181 CCAGCAACGC GGCCTTTTTA CGGTTCCTGG CCTTTTGCTG GCCTTTTGCT CACATGTTCT
     6241 TTCCTGCGTT ATCCCCTGAT TCTGTGGATA ACCGTATTAC CGCCTTTGAG TGAGCTGATA
     6301 CCGCTCGCCG CAGCCGAACG ACCGAGCGCA GCGAGTCAGT GAGCGAGGAA GCGGAAGAGC
     6361 GCCCAATACG CAAACCGCCT CTCCCCGCGC GTTGGCCGAT TCATTAATGC AGCTGGCACG
     6421 ACAGGTTTCC CGACTGGAAA GCGGGCAGTG AGCGCAACGC AATTAATGTG AGTTACCTCA
     6481 CTCATTAGGC ACCCCAGGCT TTACACTTTA TGCTTCCGGC TCCTATGTTG TGTGGAATTG
     6541 TGAGCGGATA ACAATTTCAC ACAGGAAACA GCTATGACCA TGATTACGCC AAGCGCGCAA
     6601 TTA     
//
