LOCUS       POPL241_MCP_attR        7668 bp ds-DNA     linear       04-OCT-2017
DEFINITION  .
ACCESSION   
VERSION     
SOURCE      .
  ORGANISM  .
COMMENT     
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     misc_feature    2043..2348
                     /locus_tag="ccdB"
                     /ApEinfo_fwdcolor="#ff8080"
                     /ApEinfo_revcolor="#ff8080"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2654..2893
                     /locus_tag="CYC1 terminator"
                     /ApEinfo_fwdcolor="#c0c0c0"
                     /ApEinfo_revcolor="#c0c0c0"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    385..414
                     /locus_tag="MET25 promoter?"
                     /ApEinfo_fwdcolor="#ff8080"
                     /ApEinfo_revcolor="#ff8080"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    808..932
                     /locus_tag="R1"
                     /ApEinfo_fwdcolor="#008000"
                     /ApEinfo_revcolor="#008000"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    5622..6482
                     /locus_tag="AmpR"
                     /ApEinfo_fwdcolor="#ffff80"
                     /ApEinfo_revcolor="#ffff80"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    6637..7256
                     /locus_tag="pBR322Ori"
                     /ApEinfo_fwdcolor="#808000"
                     /ApEinfo_revcolor="#808000"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    join(436..692,694..786)
                     /locus_tag="MS2 coat protein"
                     /ApEinfo_fwdcolor="#ffff00"
                     /ApEinfo_revcolor="#ffff00"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2576..2601
                     /locus_tag="3'UTR"
                     /ApEinfo_fwdcolor="#ffd1d1"
                     /ApEinfo_revcolor="#ffd1d1"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1042..1701
                     /locus_tag="cmlR"
                     /ApEinfo_fwdcolor="#808040"
                     /ApEinfo_revcolor="#808040"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2389..2511
                     /locus_tag="R2"
                     /ApEinfo_fwdcolor="#80ff00"
                     /ApEinfo_revcolor="#80ff00"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3691..4491
                     /locus_tag="New Feature"
                     /ApEinfo_fwdcolor="#008080"
                     /ApEinfo_revcolor="#008080"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    808..819
                     /locus_tag="Primer1"
                     /ApEinfo_fwdcolor="#ffff80"
                     /ApEinfo_revcolor="#ffff80"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2502..2511
                     /locus_tag="Primer2"
                     /ApEinfo_fwdcolor="#ffff80"
                     /ApEinfo_revcolor="#ffff80"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2557..2559
                     /locus_tag="Stop Codon"
                     /ApEinfo_fwdcolor="#ff0000"
                     /ApEinfo_revcolor="#ff0000"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    421..423
                     /locus_tag="Start Codon"
                     /ApEinfo_fwdcolor="#ff0000"
                     /ApEinfo_revcolor="#ff0000"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2602..2620
                     /locus_tag="MS2 hairpin"
                     /ApEinfo_fwdcolor="#fc293f"
                     /ApEinfo_revcolor="#fc293f"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2621..2640
                     /locus_tag="3'UTR(1)"
                     /ApEinfo_label="3'UTR"
                     /ApEinfo_fwdcolor="#ffd1d1"
                     /ApEinfo_revcolor="#ffd1d1"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2533..2556
                     /locus_tag="FLAG"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="cyan"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
ORIGIN
        1 ACCCTCACTA AAGGGAACAA AAGCTGGAGC TCCGGATGCA AGGGTTCGAA TCCCTTAGCT
       61 CTCATTATTT TTTGCTTTTT CTCTTGAGGT CACATGATCG CAAAATGGCA AATGGCACGT
      121 GAAGCTGTCG ATATTGGGGA ACTGTGGTGG TTGGCAAATG ACTAATTAAG TTAGTCAAGG
      181 CGCCATCCTC ATGAAAACTG TGTAACATAA TAACCGAAGT GTCGAAAAGG TGGCACCTTG
      241 TCCAATTGAA CACGCTCGAT GAAAAAAATA AGATATATAT AAGGTTAAGT AAAGCGTCTG
      301 TTAGAAAGGA AGTTTTTCCT TTTTCTTGCT CTCTTGTCTT TTCATCTACT ATTTCCTTCG
      361 TGTAATACAG GGTCGTCAGA TACATAGATA CAATTCTATT ACCCCCATCC ATACTCTAGA
      421 ATGCTAGCCG TTAAAATGGC TTCTAACTTT ACTCAGTTCG TTCTCGTCGA CAATGGCGGA
      481 ACTGGCGACG TGACTGTCGC CCCAAGCAAC TTCGCTAACG GGATCGCTGA ATGGATCAGC
      541 TCTAACTCGC GTTCACAGGC TTACAAAGTA ACCTGTAGCG TTCGTCAGAG CTCTGCGCAG
      601 AATCGCAAAT ACACCATCAA AGTCGAGGTG CCTAAAGGCG CCTGGCGTTC GTACTTAAAT
      661 ATGGAACTAA CCATTCCAAT TTTCGCCACG AATTCCGACT GCGAGCTTAT TGTTAAGGCA
      721 ATGCAAGGTC TCCTAAAAGA TGGAAACCCG ATTCCCTCAG CAATCGCAGC AAACTCCGGC
      781 ATCTACGCGG ATCCACCGGT CGCCACCACA AGTTTGTACA AAAAAGCTGA ACGAGAAACG
      841 TAAAATGATA TAAATATCAA TATATTAAAT TAGATTTTGC ATAAAAAACA GACTACATAA
      901 TACTGTAAAA CACAACATAT CCAGTCACTA TGGCGGCCGC ATTAGGGCAC CCCAGGCTTT
      961 ACACTTTATG CTTCCGGCTC GTATAATGTG TGGATTTTGA GTTAGGATCC GTCGGGATTT
     1021 TCAGGAGCTA AGGAAGCTAA AATGGAGAAA AAAATCACTG GATATACCAC CGTTGATATA
     1081 TCCCAATGGC ATCGTAAAGA ACATTTTGAG GCATTTCAGT CAGTTGCTCA ATGTACCTAT
     1141 AACCAGACCG TTCAGCTGGA TATTACGGCC TTTTTAAAGA CCGTAAAGAA AAATAAGCAC
     1201 AAGTTTTATC CGGCCTTTAT TCACATTCTT GCCCGCCTGA TGAATGCTCA TCCGGAATTC
     1261 CGTATGGCAA TGAAAGACGG TGAGCTGGTG ATATGGGATA GTGTTCACCC TTGTTACACC
     1321 GTTTTCCATG AGCAAACTGA AACGTTTTCA TCGCTCTGGA GTGAATACCA CGACGATTTC
     1381 CGGCAGTTTC TACACATATA TTCGCAAGAT GTGGCGTGTT ACGGTGAAAA CCTGGCCTAT
     1441 TTCCCTAAAG GGTTTATTGA GAATATGTTT TTCGTCTCAG CCAATCCCTG GGTGAGTTTC
     1501 ACCAGTTTTG ATTTAAACGT GGCCAATATG GACAACTTCT TCGCCCCCGT TTTCACCATG
     1561 GGCAAATATT ATACGCAAGG CGACAAGGTG CTGATGCCGC TGGCGATTCA GGTTCATCAT
     1621 GCCGTCTGTG ATGGCTTCCA TGTCGGCAGA ATGCTTAATG AATTACAACA GTACTGCGAT
     1681 GAGTGGCAGG GCGGGGCGTA ATCTAGAGGA TCCGGCTTAC TAAAAGCCAG ATAACAGTAT
     1741 GCGTATTTGC GCGCTGATTT TTGCGGTATA AGAATATATA CTGATATGTA TACCCGAAGT
     1801 ATGTCAAAAA GAGGTGTGCT ATGAAGCAGC GTATTACAGT GACAGTTGAC AGCGACAGCT
     1861 ATCAGTTGCT CAAGGCATAT ATGATGTCAA TATCTCCGGT CTGGTAAGCA CAACCATGCA
     1921 GAATGAAGCC CGTCGTCTGC GTGCCGAACG CTGGAAAGCG GAAAATCAGG AAGGGATGGC
     1981 TGAGGTCGCC CGGTTTATTG AAATGAACGG CTCTTTTGCT GACGAGAACA GGGACTGGTG
     2041 AAATGCAGTT TAAGGTTTAC ACCTATAAAA GAGAGAGCCG TTATCGTCTG TTTGTGGATG
     2101 TACAGAGTGA TATTATTGAC ACGCCCGGGC GACGGATGGT GATCCCCCTG GCCAGTGCAC
     2161 GTCTGCTGTC AGATAAAGTC CCCCGTGAAC TTTACCCGGT GGTGCATATC GGGGATGAAA
     2221 GCTGGCGCAT GATGACCACC GATATGGCCA GTGTGCCGGT CTCCGTTATC GGGGAAGAAG
     2281 TGGCTGATCT CAGCCACCGC GAAAATGACA TCAAAAACGC CATTAACCTG ATGTTCTGGG
     2341 GAATATAAAT GTCAGGCTCC CTTATACACA GCCAGTCTGC AGGTCGACCA TAGTGACTGG
     2401 ATATGTTGTG TTTTACAGTA TTATGTAGTC TGTTTTTTAT GCAAAATCTA ATTTAATATA
     2461 TTGATATTTA TATCATTTTA CGTTTCTCGT TCAGCTTTCT TGTACAAAGT GGTTTCAAGA
     2521 GGGCCCTTCG AAGATTATAA AGACGATGAC GATAAGTAAA TCGATACCGT CGACGATCCT
     2581 ACGGTACTTA TTGCCAAGAA AGCACGAGCA TCAGCCGTGC CTCCAGGTCG AATCTTCAAA
     2641 CTCGAGTCAT GTAATTAGTT ATGTCACGCT TACATTCACG CCCTCCCCCC ACATCCGCTC
     2701 TAACCGAAAA GGAAGGAGTT AGACAACCTG AAGTCTAGGT CCCTATTTAT TTTTTTATAG
     2761 TTATGTTAGT ATTAAGAACG TTATTTATAT TTCAAATTTT TCTTTTTTTT CTGTACAGAC
     2821 GCGTGTACGC ATGTAACATT ATACTGAAAA CCTTGCTTGA GAAGGTTTTG GGACGCTCGA
     2881 AGGCTTTAAT TTGCGGCCGG TACCCAATTC GCCCTATAGT GAGTCGTATT ACGCGCGCTC
     2941 ACTGGCCGTC GTTTTACAAC GTCGTGACTG GGAAAACCCT GGCGTTACCC AACTTAATCG
     3001 CCTTGCAGCA CATCCCCCTT TCGCCAGCTG GCGTAATAGC GAAGAGGCCC GCACCGATCG
     3061 CCCTTCCCAA CAGTTGCGCA GCCTGAATGG CGAATGGCGC GACGCGCCCT GTAGCGGCGC
     3121 ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT
     3181 AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG
     3241 TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA
     3301 CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT
     3361 TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG
     3421 AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC
     3481 GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TTAACAAAAT
     3541 ATTAACGTTT ACAATTTCCT GATGCGGTAT TTTCTCCTTA CGCATCTGTG CGGTATTTCA
     3601 CACCGCATAG GGTAATAACT GATATAATTA AATTGAAGCT CTAATTTGTG AGTTTAGTAT
     3661 ACATGCATTT ACTTATAATA CAGTTTTTTA GTTTTGCTGG CCGCATCTTC TCAAATATGC
     3721 TTCCCAGCCT GCTTTTCTGT AACGTTCACC CTCTACCTTA GCATCCCTTC CCTTTGCAAA
     3781 TAGTCCTCTT CCAACAATAA TAATGTCAGA TCCTGTAGAG ACCACATCAT CCACGGTTCT
     3841 ATACTGTTGA CCCAATGCGT CTCCCTTGTC ATCTAAACCC ACACCGGGTG TCATAATCAA
     3901 CCAATCGTAA CCTTCATCTC TTCCACCCAT GTCTCTTTGA GCAATAAAGC CGATAACAAA
     3961 ATCTTTGTCG CTCTTCGCAA TGTCAACAGT ACCCTTAGTA TATTCTCCAG TAGATAGGGA
     4021 GCCCTTGCAT GACAATTCTG CTAACATCAA AAGGCCTCTA GGTTCCTTTG TTACTTCTTC
     4081 TGCCGCCTGC TTCAAACCGC TAACAATACC TGGGCCCACC ACACCGTGTG CATTCGTAAT
     4141 GTCTGCCCAT TCTGCTATTC TGTATACACC CGCAGAGTAC TGCAATTTGA CTGTATTACC
     4201 AATGTCAGCA AATTTTCTGT CTTCGAAGAG TAAAAAATTG TACTTGGCGG ATAATGCCTT
     4261 TAGCGGCTTA ACTGTGCCCT CCATGGAAAA ATCAGTCAAG ATATCCACAT GTGTTTTTAG
     4321 TAAACAAATT TTGGGACCTA ATGCTTCAAC TAACTCCAGT AATTCCTTGG TGGTACGAAC
     4381 ATCCAATGAA GCACACAAGT TTGTTTGCTT TTCGTGCATG ATATTAAATA GCTTGGCAGC
     4441 AACAGGACTA GGATGAGTAG CAGCACGTTC CTTATATGTA GCTTTCGACA TGATTTATCT
     4501 TCGTTTCCTG CAGGTTTTTG TTCTGTGCAG TTGGGTTAAG AATACTGGGC AATTTCATGT
     4561 TTCTTCAACA CTACATATGC GTATATATAC CAATCTAAGT CTGTGCTCCT TCCTTCGTTC
     4621 TTCCTTCTGT TCGGAGATTA CCGAATCAAA AAAATTTCAA AGAAACCGAA ATCAAAAAAA
     4681 AGAATAAAAA AAAAATGATG AATTGAATTG AAAAGCTGTG GTATGGTGCA CTCTCAGTAC
     4741 AATCTGCTCT GATGCCGCAT AGTTAAGCCA GCCCCGACAC CCGCCAACAC CCGCTGACGC
     4801 GCCCTGACGG GCTTGTCTGC TCCCGGCATC CGCTTACAGA CAAGCTGTGA CCGTCTCCGG
     4861 GAGCTGCATG TGTCAGAGGT TTTCACCGTC ATCACCGAAA CGCGCGAGAC GAAAGGGCCT
     4921 CGTGATACGC CTATTTTTAT AGGTTAATGT CATGATAATA ATGGTTTCTT AGGACGGATC
     4981 GCTTGCCTGT AACTTACACG CGCCTCGTAT CTTTTAATGA TGGAATAATT TGGGAATTTA
     5041 CTCTGTGTTT ATTTATTTTT ATGTTTTGTA TTTGGATTTT AGAAAGTAAA TAAAGAAGGT
     5101 AGAAGAGTTA CGGAATGAAG AAAAAAAAAT AAACAAAGGT TTAAAAAATT TCAACAAAAA
     5161 GCGTACTTTA CATATATATT TATTAGACAA GAAAAGCAGA TTAAATAGAT ATACATTCGA
     5221 TTAACGATAA GTAAAATGTA AAATCACAGG ATTTTCGTGT GTGGTCTTCT ACACAGACAA
     5281 GATGAAACAA TTCGGCATTA ATACCTGAGA GCAGGAAGAG CAAGATAAAA GGTAGTATTT
     5341 GTTGGCGATC CCCCTAGAGT CTTTTACATC TTCGGAAAAC AAAAACTATT TTTTCTTTAA
     5401 TTTCTTTTTT TACTTTCTAT TTTTAATTTA TATATTTATA TTAAAAAATT TAAATTATAA
     5461 TTATTTTTAT AGCACGTGAT GAAAAGGACC CAGGTGGCAC TTTTCGGGGA AATGTGCGCG
     5521 GAACCCCTAT TTGTTTATTT TTCTAAATAC ATTCAAATAT GTATCCGCTC ATGAGACAAT
     5581 AACCCTGATA AATGCTTCAA TAATATTGAA AAAGGAAGAG TATGAGTATT CAACATTTCC
     5641 GTGTCGCCCT TATTCCCTTT TTTGCGGCAT TTTGCCTTCC TGTTTTTGCT CACCCAGAAA
     5701 CGCTGGTGAA AGTAAAAGAT GCTGAAGATC AGTTGGGTGC ACGAGTGGGT TACATCGAAC
     5761 TGGATCTCAA CAGCGGTAAG ATCCTTGAGA GTTTTCGCCC CGAAGAACGT TTTCCAATGA
     5821 TGAGCACTTT TAAAGTTCTG CTATGTGGCG CGGTATTATC CCGTATTGAC GCCGGGCAAG
     5881 AGCAACTCGG TCGCCGCATA CACTATTCTC AGAATGACTT GGTTGAGTAC TCACCAGTCA
     5941 CAGAAAAGCA TCTTACGGAT GGCATGACAG TAAGAGAATT ATGCAGTGCT GCCATAACCA
     6001 TGAGTGATAA CACTGCGGCC AACTTACTTC TGACAACGAT CGGAGGACCG AAGGAGCTAA
     6061 CCGCTTTTTT GCACAACATG GGGGATCATG TAACTCGCCT TGATCGTTGG GAACCGGAGC
     6121 TGAATGAAGC CATACCAAAC GACGAGCGTG ACACCACGAT GCCTGTAGCA ATGGCAACAA
     6181 CGTTGCGCAA ACTATTAACT GGCGAACTAC TTACTCTAGC TTCCCGGCAA CAATTAATAG
     6241 ACTGGATGGA GGCGGATAAA GTTGCAGGAC CACTTCTGCG CTCGGCCCTT CCGGCTGGCT
     6301 GGTTTATTGC TGATAAATCT GGAGCCGGTG AGCGTGGGTC TCGCGGTATC ATTGCAGCAC
     6361 TGGGGCCAGA TGGTAAGCCC TCCCGTATCG TAGTTATCTA CACGACGGGG AGTCAGGCAA
     6421 CTATGGATGA ACGAAATAGA CAGATCGCTG AGATAGGTGC CTCACTGATT AAGCATTGGT
     6481 AACTGTCAGA CCAAGTTTAC TCATATATAC TTTAGATTGA TTTAAAACTT CATTTTTAAT
     6541 TTAAAAGGAT CTAGGTGAAG ATCCTTTTTG ATAATCTCAT GACCAAAATC CCTTAACGTG
     6601 AGTTTTCGTT CCACTGAGCG TCAGACCCCG TAGAAAAGAT CAAAGGATCT TCTTGAGATC
     6661 CTTTTTTTCT GCGCGTAATC TGCTGCTTGC AAACAAAAAA ACCACCGCTA CCAGCGGTGG
     6721 TTTGTTTGCC GGATCAAGAG CTACCAACTC TTTTTCCGAA GGTAACTGGC TTCAGCAGAG
     6781 CGCAGATACC AAATACTGTC CTTCTAGTGT AGCCGTAGTT AGGCCACCAC TTCAAGAACT
     6841 CTGTAGCACC GCCTACATAC CTCGCTCTGC TAATCCTGTT ACCAGTGGCT GCTGCCAGTG
     6901 GCGATAAGTC GTGTCTTACC GGGTTGGACT CAAGACGATA GTTACCGGAT AAGGCGCAGC
     6961 GGTCGGGCTG AACGGGGGGT TCGTGCACAC AGCCCAGCTT GGAGCGAACG ACCTACACCG
     7021 AACTGAGATA CCTACAGCGT GAGCTATGAG AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG
     7081 CGGACAGGTA TCCGGTAAGC GGCAGGGTCG GAACAGGAGA GCGCACGAGG GAGCTTCCAG
     7141 GGGGAAACGC CTGGTATCTT TATAGTCCTG TCGGGTTTCG CCACCTCTGA CTTGAGCGTC
     7201 GATTTTTGTG ATGCTCGTCA GGGGGGCGGA GCCTATGGAA AAACGCCAGC AACGCGGCCT
     7261 TTTTACGGTT CCTGGCCTTT TGCTGGCCTT TTGCTCACAT GTTCTTTCCT GCGTTATCCC
     7321 CTGATTCTGT GGATAACCGT ATTACCGCCT TTGAGTGAGC TGATACCGCT CGCCGCAGCC
     7381 GAACGACCGA GCGCAGCGAG TCAGTGAGCG AGGAAGCGGA AGAGCGCCCA ATACGCAAAC
     7441 CGCCTCTCCC CGCGCGTTGG CCGATTCATT AATGCAGCTG GCACGACAGG TTTCCCGACT
     7501 GGAAAGCGGG CAGTGAGCGC AACGCAATTA ATGTGAGTTA CCTCACTCAT TAGGCACCCC
     7561 AGGCTTTACA CTTTATGCTT CCGGCTCCTA TGTTGTGTGG AATTGTGAGC GGATAACAAT
     7621 TTCACACAGG AAACAGCTAT GACCATGATT ACGCCAAGCG CGCAATTA 
//
