LOCUS       POPL241_MCP_attR        7674 bp ds-DNA     linear       04-OCT-2017
DEFINITION  .
ACCESSION   
VERSION     
SOURCE      .
  ORGANISM  .
COMMENT     
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     misc_feature    2043..2348
                     /locus_tag="ccdB"
                     /ApEinfo_fwdcolor="#ff8080"
                     /ApEinfo_revcolor="#ff8080"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2660..2899
                     /locus_tag="CYC1 terminator"
                     /ApEinfo_fwdcolor="#c0c0c0"
                     /ApEinfo_revcolor="#c0c0c0"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    385..414
                     /locus_tag="MET25 promoter?"
                     /ApEinfo_fwdcolor="#ff8080"
                     /ApEinfo_revcolor="#ff8080"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    808..932
                     /locus_tag="R1"
                     /ApEinfo_fwdcolor="#008000"
                     /ApEinfo_revcolor="#008000"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    5628..6488
                     /locus_tag="AmpR"
                     /ApEinfo_fwdcolor="#ffff80"
                     /ApEinfo_revcolor="#ffff80"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    6643..7262
                     /locus_tag="pBR322Ori"
                     /ApEinfo_fwdcolor="#808000"
                     /ApEinfo_revcolor="#808000"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    join(436..692,694..786)
                     /locus_tag="MS2 coat protein"
                     /ApEinfo_fwdcolor="#ffff00"
                     /ApEinfo_revcolor="#ffff00"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2582..2607
                     /locus_tag="3'UTR"
                     /ApEinfo_fwdcolor="#ffd1d1"
                     /ApEinfo_revcolor="#ffd1d1"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1042..1701
                     /locus_tag="cmlR"
                     /ApEinfo_fwdcolor="#808040"
                     /ApEinfo_revcolor="#808040"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2389..2511
                     /locus_tag="R2"
                     /ApEinfo_fwdcolor="#80ff00"
                     /ApEinfo_revcolor="#80ff00"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3697..4497
                     /locus_tag="New Feature"
                     /ApEinfo_fwdcolor="#008080"
                     /ApEinfo_revcolor="#008080"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    808..819
                     /locus_tag="Primer1"
                     /ApEinfo_fwdcolor="#ffff80"
                     /ApEinfo_revcolor="#ffff80"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2502..2511
                     /locus_tag="Primer2"
                     /ApEinfo_fwdcolor="#ffff80"
                     /ApEinfo_revcolor="#ffff80"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2563..2565
                     /locus_tag="Stop Codon"
                     /ApEinfo_fwdcolor="#ff0000"
                     /ApEinfo_revcolor="#ff0000"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2533..2562
                     /locus_tag="mycTag"
                     /ApEinfo_fwdcolor="#8000ff"
                     /ApEinfo_revcolor="#8000ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    421..423
                     /locus_tag="Start Codon"
                     /ApEinfo_fwdcolor="#ff0000"
                     /ApEinfo_revcolor="#ff0000"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2608..2626
                     /locus_tag="MS2 hairpin"
                     /ApEinfo_fwdcolor="#fc293f"
                     /ApEinfo_revcolor="#fc293f"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2627..2646
                     /locus_tag="3'UTR(1)"
                     /ApEinfo_label="3'UTR"
                     /ApEinfo_fwdcolor="#ffd1d1"
                     /ApEinfo_revcolor="#ffd1d1"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
ORIGIN
        1 ACCCTCACTA AAGGGAACAA AAGCTGGAGC TCCGGATGCA AGGGTTCGAA TCCCTTAGCT
       61 CTCATTATTT TTTGCTTTTT CTCTTGAGGT CACATGATCG CAAAATGGCA AATGGCACGT
      121 GAAGCTGTCG ATATTGGGGA ACTGTGGTGG TTGGCAAATG ACTAATTAAG TTAGTCAAGG
      181 CGCCATCCTC ATGAAAACTG TGTAACATAA TAACCGAAGT GTCGAAAAGG TGGCACCTTG
      241 TCCAATTGAA CACGCTCGAT GAAAAAAATA AGATATATAT AAGGTTAAGT AAAGCGTCTG
      301 TTAGAAAGGA AGTTTTTCCT TTTTCTTGCT CTCTTGTCTT TTCATCTACT ATTTCCTTCG
      361 TGTAATACAG GGTCGTCAGA TACATAGATA CAATTCTATT ACCCCCATCC ATACTCTAGA
      421 ATGCTAGCCG TTAAAATGGC TTCTAACTTT ACTCAGTTCG TTCTCGTCGA CAATGGCGGA
      481 ACTGGCGACG TGACTGTCGC CCCAAGCAAC TTCGCTAACG GGATCGCTGA ATGGATCAGC
      541 TCTAACTCGC GTTCACAGGC TTACAAAGTA ACCTGTAGCG TTCGTCAGAG CTCTGCGCAG
      601 AATCGCAAAT ACACCATCAA AGTCGAGGTG CCTAAAGGCG CCTGGCGTTC GTACTTAAAT
      661 ATGGAACTAA CCATTCCAAT TTTCGCCACG AATTCCGACT GCGAGCTTAT TGTTAAGGCA
      721 ATGCAAGGTC TCCTAAAAGA TGGAAACCCG ATTCCCTCAG CAATCGCAGC AAACTCCGGC
      781 ATCTACGCGG ATCCACCGGT CGCCACCACA AGTTTGTACA AAAAAGCTGA ACGAGAAACG
      841 TAAAATGATA TAAATATCAA TATATTAAAT TAGATTTTGC ATAAAAAACA GACTACATAA
      901 TACTGTAAAA CACAACATAT CCAGTCACTA TGGCGGCCGC ATTAGGGCAC CCCAGGCTTT
      961 ACACTTTATG CTTCCGGCTC GTATAATGTG TGGATTTTGA GTTAGGATCC GTCGGGATTT
     1021 TCAGGAGCTA AGGAAGCTAA AATGGAGAAA AAAATCACTG GATATACCAC CGTTGATATA
     1081 TCCCAATGGC ATCGTAAAGA ACATTTTGAG GCATTTCAGT CAGTTGCTCA ATGTACCTAT
     1141 AACCAGACCG TTCAGCTGGA TATTACGGCC TTTTTAAAGA CCGTAAAGAA AAATAAGCAC
     1201 AAGTTTTATC CGGCCTTTAT TCACATTCTT GCCCGCCTGA TGAATGCTCA TCCGGAATTC
     1261 CGTATGGCAA TGAAAGACGG TGAGCTGGTG ATATGGGATA GTGTTCACCC TTGTTACACC
     1321 GTTTTCCATG AGCAAACTGA AACGTTTTCA TCGCTCTGGA GTGAATACCA CGACGATTTC
     1381 CGGCAGTTTC TACACATATA TTCGCAAGAT GTGGCGTGTT ACGGTGAAAA CCTGGCCTAT
     1441 TTCCCTAAAG GGTTTATTGA GAATATGTTT TTCGTCTCAG CCAATCCCTG GGTGAGTTTC
     1501 ACCAGTTTTG ATTTAAACGT GGCCAATATG GACAACTTCT TCGCCCCCGT TTTCACCATG
     1561 GGCAAATATT ATACGCAAGG CGACAAGGTG CTGATGCCGC TGGCGATTCA GGTTCATCAT
     1621 GCCGTCTGTG ATGGCTTCCA TGTCGGCAGA ATGCTTAATG AATTACAACA GTACTGCGAT
     1681 GAGTGGCAGG GCGGGGCGTA ATCTAGAGGA TCCGGCTTAC TAAAAGCCAG ATAACAGTAT
     1741 GCGTATTTGC GCGCTGATTT TTGCGGTATA AGAATATATA CTGATATGTA TACCCGAAGT
     1801 ATGTCAAAAA GAGGTGTGCT ATGAAGCAGC GTATTACAGT GACAGTTGAC AGCGACAGCT
     1861 ATCAGTTGCT CAAGGCATAT ATGATGTCAA TATCTCCGGT CTGGTAAGCA CAACCATGCA
     1921 GAATGAAGCC CGTCGTCTGC GTGCCGAACG CTGGAAAGCG GAAAATCAGG AAGGGATGGC
     1981 TGAGGTCGCC CGGTTTATTG AAATGAACGG CTCTTTTGCT GACGAGAACA GGGACTGGTG
     2041 AAATGCAGTT TAAGGTTTAC ACCTATAAAA GAGAGAGCCG TTATCGTCTG TTTGTGGATG
     2101 TACAGAGTGA TATTATTGAC ACGCCCGGGC GACGGATGGT GATCCCCCTG GCCAGTGCAC
     2161 GTCTGCTGTC AGATAAAGTC CCCCGTGAAC TTTACCCGGT GGTGCATATC GGGGATGAAA
     2221 GCTGGCGCAT GATGACCACC GATATGGCCA GTGTGCCGGT CTCCGTTATC GGGGAAGAAG
     2281 TGGCTGATCT CAGCCACCGC GAAAATGACA TCAAAAACGC CATTAACCTG ATGTTCTGGG
     2341 GAATATAAAT GTCAGGCTCC CTTATACACA GCCAGTCTGC AGGTCGACCA TAGTGACTGG
     2401 ATATGTTGTG TTTTACAGTA TTATGTAGTC TGTTTTTTAT GCAAAATCTA ATTTAATATA
     2461 TTGATATTTA TATCATTTTA CGTTTCTCGT TCAGCTTTCT TGTACAAAGT GGTTTCAAGA
     2521 GGGCCCTTCG AAGAACAAAA ACTAATCTCA GAAGAGGATC TGTAAATCGA TACCGTCGAC
     2581 GATCCTACGG TACTTATTGC CAAGAAAGCA CGAGCATCAG CCGTGCCTCC AGGTCGAATC
     2641 TTCAAACTCG AGTCATGTAA TTAGTTATGT CACGCTTACA TTCACGCCCT CCCCCCACAT
     2701 CCGCTCTAAC CGAAAAGGAA GGAGTTAGAC AACCTGAAGT CTAGGTCCCT ATTTATTTTT
     2761 TTATAGTTAT GTTAGTATTA AGAACGTTAT TTATATTTCA AATTTTTCTT TTTTTTCTGT
     2821 ACAGACGCGT GTACGCATGT AACATTATAC TGAAAACCTT GCTTGAGAAG GTTTTGGGAC
     2881 GCTCGAAGGC TTTAATTTGC GGCCGGTACC CAATTCGCCC TATAGTGAGT CGTATTACGC
     2941 GCGCTCACTG GCCGTCGTTT TACAACGTCG TGACTGGGAA AACCCTGGCG TTACCCAACT
     3001 TAATCGCCTT GCAGCACATC CCCCTTTCGC CAGCTGGCGT AATAGCGAAG AGGCCCGCAC
     3061 CGATCGCCCT TCCCAACAGT TGCGCAGCCT GAATGGCGAA TGGCGCGACG CGCCCTGTAG
     3121 CGGCGCATTA AGCGCGGCGG GTGTGGTGGT TACGCGCAGC GTGACCGCTA CACTTGCCAG
     3181 CGCCCTAGCG CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT CTCGCCACGT TCGCCGGCTT
     3241 TCCCCGTCAA GCTCTAAATC GGGGGCTCCC TTTAGGGTTC CGATTTAGTG CTTTACGGCA
     3301 CCTCGACCCC AAAAAACTTG ATTAGGGTGA TGGTTCACGT AGTGGGCCAT CGCCCTGATA
     3361 GACGGTTTTT CGCCCTTTGA CGTTGGAGTC CACGTTCTTT AATAGTGGAC TCTTGTTCCA
     3421 AACTGGAACA ACACTCAACC CTATCTCGGT CTATTCTTTT GATTTATAAG GGATTTTGCC
     3481 GATTTCGGCC TATTGGTTAA AAAATGAGCT GATTTAACAA AAATTTAACG CGAATTTTAA
     3541 CAAAATATTA ACGTTTACAA TTTCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT
     3601 ATTTCACACC GCATAGGGTA ATAACTGATA TAATTAAATT GAAGCTCTAA TTTGTGAGTT
     3661 TAGTATACAT GCATTTACTT ATAATACAGT TTTTTAGTTT TGCTGGCCGC ATCTTCTCAA
     3721 ATATGCTTCC CAGCCTGCTT TTCTGTAACG TTCACCCTCT ACCTTAGCAT CCCTTCCCTT
     3781 TGCAAATAGT CCTCTTCCAA CAATAATAAT GTCAGATCCT GTAGAGACCA CATCATCCAC
     3841 GGTTCTATAC TGTTGACCCA ATGCGTCTCC CTTGTCATCT AAACCCACAC CGGGTGTCAT
     3901 AATCAACCAA TCGTAACCTT CATCTCTTCC ACCCATGTCT CTTTGAGCAA TAAAGCCGAT
     3961 AACAAAATCT TTGTCGCTCT TCGCAATGTC AACAGTACCC TTAGTATATT CTCCAGTAGA
     4021 TAGGGAGCCC TTGCATGACA ATTCTGCTAA CATCAAAAGG CCTCTAGGTT CCTTTGTTAC
     4081 TTCTTCTGCC GCCTGCTTCA AACCGCTAAC AATACCTGGG CCCACCACAC CGTGTGCATT
     4141 CGTAATGTCT GCCCATTCTG CTATTCTGTA TACACCCGCA GAGTACTGCA ATTTGACTGT
     4201 ATTACCAATG TCAGCAAATT TTCTGTCTTC GAAGAGTAAA AAATTGTACT TGGCGGATAA
     4261 TGCCTTTAGC GGCTTAACTG TGCCCTCCAT GGAAAAATCA GTCAAGATAT CCACATGTGT
     4321 TTTTAGTAAA CAAATTTTGG GACCTAATGC TTCAACTAAC TCCAGTAATT CCTTGGTGGT
     4381 ACGAACATCC AATGAAGCAC ACAAGTTTGT TTGCTTTTCG TGCATGATAT TAAATAGCTT
     4441 GGCAGCAACA GGACTAGGAT GAGTAGCAGC ACGTTCCTTA TATGTAGCTT TCGACATGAT
     4501 TTATCTTCGT TTCCTGCAGG TTTTTGTTCT GTGCAGTTGG GTTAAGAATA CTGGGCAATT
     4561 TCATGTTTCT TCAACACTAC ATATGCGTAT ATATACCAAT CTAAGTCTGT GCTCCTTCCT
     4621 TCGTTCTTCC TTCTGTTCGG AGATTACCGA ATCAAAAAAA TTTCAAAGAA ACCGAAATCA
     4681 AAAAAAAGAA TAAAAAAAAA ATGATGAATT GAATTGAAAA GCTGTGGTAT GGTGCACTCT
     4741 CAGTACAATC TGCTCTGATG CCGCATAGTT AAGCCAGCCC CGACACCCGC CAACACCCGC
     4801 TGACGCGCCC TGACGGGCTT GTCTGCTCCC GGCATCCGCT TACAGACAAG CTGTGACCGT
     4861 CTCCGGGAGC TGCATGTGTC AGAGGTTTTC ACCGTCATCA CCGAAACGCG CGAGACGAAA
     4921 GGGCCTCGTG ATACGCCTAT TTTTATAGGT TAATGTCATG ATAATAATGG TTTCTTAGGA
     4981 CGGATCGCTT GCCTGTAACT TACACGCGCC TCGTATCTTT TAATGATGGA ATAATTTGGG
     5041 AATTTACTCT GTGTTTATTT ATTTTTATGT TTTGTATTTG GATTTTAGAA AGTAAATAAA
     5101 GAAGGTAGAA GAGTTACGGA ATGAAGAAAA AAAAATAAAC AAAGGTTTAA AAAATTTCAA
     5161 CAAAAAGCGT ACTTTACATA TATATTTATT AGACAAGAAA AGCAGATTAA ATAGATATAC
     5221 ATTCGATTAA CGATAAGTAA AATGTAAAAT CACAGGATTT TCGTGTGTGG TCTTCTACAC
     5281 AGACAAGATG AAACAATTCG GCATTAATAC CTGAGAGCAG GAAGAGCAAG ATAAAAGGTA
     5341 GTATTTGTTG GCGATCCCCC TAGAGTCTTT TACATCTTCG GAAAACAAAA ACTATTTTTT
     5401 CTTTAATTTC TTTTTTTACT TTCTATTTTT AATTTATATA TTTATATTAA AAAATTTAAA
     5461 TTATAATTAT TTTTATAGCA CGTGATGAAA AGGACCCAGG TGGCACTTTT CGGGGAAATG
     5521 TGCGCGGAAC CCCTATTTGT TTATTTTTCT AAATACATTC AAATATGTAT CCGCTCATGA
     5581 GACAATAACC CTGATAAATG CTTCAATAAT ATTGAAAAAG GAAGAGTATG AGTATTCAAC
     5641 ATTTCCGTGT CGCCCTTATT CCCTTTTTTG CGGCATTTTG CCTTCCTGTT TTTGCTCACC
     5701 CAGAAACGCT GGTGAAAGTA AAAGATGCTG AAGATCAGTT GGGTGCACGA GTGGGTTACA
     5761 TCGAACTGGA TCTCAACAGC GGTAAGATCC TTGAGAGTTT TCGCCCCGAA GAACGTTTTC
     5821 CAATGATGAG CACTTTTAAA GTTCTGCTAT GTGGCGCGGT ATTATCCCGT ATTGACGCCG
     5881 GGCAAGAGCA ACTCGGTCGC CGCATACACT ATTCTCAGAA TGACTTGGTT GAGTACTCAC
     5941 CAGTCACAGA AAAGCATCTT ACGGATGGCA TGACAGTAAG AGAATTATGC AGTGCTGCCA
     6001 TAACCATGAG TGATAACACT GCGGCCAACT TACTTCTGAC AACGATCGGA GGACCGAAGG
     6061 AGCTAACCGC TTTTTTGCAC AACATGGGGG ATCATGTAAC TCGCCTTGAT CGTTGGGAAC
     6121 CGGAGCTGAA TGAAGCCATA CCAAACGACG AGCGTGACAC CACGATGCCT GTAGCAATGG
     6181 CAACAACGTT GCGCAAACTA TTAACTGGCG AACTACTTAC TCTAGCTTCC CGGCAACAAT
     6241 TAATAGACTG GATGGAGGCG GATAAAGTTG CAGGACCACT TCTGCGCTCG GCCCTTCCGG
     6301 CTGGCTGGTT TATTGCTGAT AAATCTGGAG CCGGTGAGCG TGGGTCTCGC GGTATCATTG
     6361 CAGCACTGGG GCCAGATGGT AAGCCCTCCC GTATCGTAGT TATCTACACG ACGGGGAGTC
     6421 AGGCAACTAT GGATGAACGA AATAGACAGA TCGCTGAGAT AGGTGCCTCA CTGATTAAGC
     6481 ATTGGTAACT GTCAGACCAA GTTTACTCAT ATATACTTTA GATTGATTTA AAACTTCATT
     6541 TTTAATTTAA AAGGATCTAG GTGAAGATCC TTTTTGATAA TCTCATGACC AAAATCCCTT
     6601 AACGTGAGTT TTCGTTCCAC TGAGCGTCAG ACCCCGTAGA AAAGATCAAA GGATCTTCTT
     6661 GAGATCCTTT TTTTCTGCGC GTAATCTGCT GCTTGCAAAC AAAAAAACCA CCGCTACCAG
     6721 CGGTGGTTTG TTTGCCGGAT CAAGAGCTAC CAACTCTTTT TCCGAAGGTA ACTGGCTTCA
     6781 GCAGAGCGCA GATACCAAAT ACTGTCCTTC TAGTGTAGCC GTAGTTAGGC CACCACTTCA
     6841 AGAACTCTGT AGCACCGCCT ACATACCTCG CTCTGCTAAT CCTGTTACCA GTGGCTGCTG
     6901 CCAGTGGCGA TAAGTCGTGT CTTACCGGGT TGGACTCAAG ACGATAGTTA CCGGATAAGG
     6961 CGCAGCGGTC GGGCTGAACG GGGGGTTCGT GCACACAGCC CAGCTTGGAG CGAACGACCT
     7021 ACACCGAACT GAGATACCTA CAGCGTGAGC TATGAGAAAG CGCCACGCTT CCCGAAGGGA
     7081 GAAAGGCGGA CAGGTATCCG GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC
     7141 TTCCAGGGGG AAACGCCTGG TATCTTTATA GTCCTGTCGG GTTTCGCCAC CTCTGACTTG
     7201 AGCGTCGATT TTTGTGATGC TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC GCCAGCAACG
     7261 CGGCCTTTTT ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC TCACATGTTC TTTCCTGCGT
     7321 TATCCCCTGA TTCTGTGGAT AACCGTATTA CCGCCTTTGA GTGAGCTGAT ACCGCTCGCC
     7381 GCAGCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA AGCGGAAGAG CGCCCAATAC
     7441 GCAAACCGCC TCTCCCCGCG CGTTGGCCGA TTCATTAATG CAGCTGGCAC GACAGGTTTC
     7501 CCGACTGGAA AGCGGGCAGT GAGCGCAACG CAATTAATGT GAGTTACCTC ACTCATTAGG
     7561 CACCCCAGGC TTTACACTTT ATGCTTCCGG CTCCTATGTT GTGTGGAATT GTGAGCGGAT
     7621 AACAATTTCA CACAGGAAAC AGCTATGACC ATGATTACGC CAAGCGCGCA ATTA
//
